inoculate with造句

• Root nodules of the soybean and alfalfa inoculated with xj96077 ( g ) and colony of xj96077 ( g ) were observed using the confocal laser scanning microscope , and the expressions of gfp were detected
  而且，通过不同品种的苜蓿、大豆的结瘤试验，发现xj96077对不同大豆品种的结瘤能力不同。
• Using the v . harveyi luminescence bioassay , expression of the luxs gene was detected in transformed tobacco . induced defence responses were observed after inoculated with plant viruses
  利用哈氏弧菌生物发光实验检测到该基因在烟草中得到表达，并通过病毒接种实验观测到转化烟草被诱导的抗性反应。
• Influences on chloroplasts " structure and chlorophyll concentration of host plants by tumv infection host plants began to take on systemic mosaic symptoms in the 15th day after having been inoculated with tumv jc - 1
  Tumv侵染对叶绿体结构和叶绿素含量的影响接种tumvjc - 115d ，青菜和芥菜开始表现系统花叶，其叶绿体结构和叶绿素含量也发生了相应变化。
• One of the first inklings that viruses could be useful in combating cancer came in 1912 , when an italian gynecologist observed the regression of cervical cancer in a woman who was inoculated with a rabies vaccine made from a live , crippled form of the rabies virus
  最早显示病毒可能可以用来对抗癌症的迹象之一，于1912年浮现：有位义大利妇科医生观察到某个妇女在注射了减毒狂犬病疫苗之后，其子宫颈癌有消退的现象。
• Ing time of 55 pumpkin materials which were inoculated with wmv - 2 . the result is : 33 materials are s , 17 materials are mr , 5 materials are r . this result provides test materials for disease resistance breeding . surveying and de terming the conductirity , chlorophy content and ppo
  利用上述研究结果对55份南瓜材料接种wmv - 2进行苗期抗病性鉴定，结果有33份感病材料， 17份中抗材料， 5份抗病材料，为抗病毒育种提供了试验材料。
• Sudies on the trannsmission of phytophthora root rot of soybean had been carriedout with the soil debris and the seeds which came from natural diseased fields as well as thesoil and seeds which inoculated with the pathogen
  对带菌土壤、病残体进行传病试验，研究了人工接种条件下种子带菌条件及带菌种子传病情况，结果表明，带菌土壤和病残体无论是在当年还是越冬后都可有效地传播大豆疫霉根腐病，是大豆疫霉根腐病的主要初侵染源和传播途径。
• In contrast , the specific antibodies induced by orf4 , orf5 or orf6 appeared later and the titers of elisa antibodies were lower too . after the fourth inoculation , the specific antibodies could be detected by wb in all piglets inoculated with recombinant plasmids
  而带有prrsvorf4 、 orf5或orf6的重组质粒诱导猪体产生抗体的速度较慢， elisa抗体滴度较低，有的动物在4次免疫后都没有检测到博士学位论文中文拘要中国农科院研究生院elrsa抗体。
• The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal , nosal routes or via the allantoic cavity route , and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated
  本试验全面而系统地观察了传染性法氏囊病病毒变异e株，通过泄殖腔、鼻腔和尿囊腔接种雏鸡和鸡胚后不同时间法氏囊的组织形态学变化。
• The results also indicated that the virulence of the most ibdv field collected in recent years was between the typical very virulent and standard virulent strains . a very virulent strain gx8 / 99 of ibdv was studied for its mortality in spf chickens and commercial egg - type chickens at different age inoculated with bursal suspension of 20 - 2000 eld50 in repeated experiments
  选择致病性最强的来自广西的gx8 99株，在对法氏囊悬液中病毒用鸡胚半数致死量( eld _ ( 50 ) )定量测定后，在不同年龄的spf鸡和商品代蛋用型坞多次重复地比较研究了该毒株的致病性。
• Tongbai county of henan province is one of the high - epidemic areas of bovine theileriosis . in 1985 , gelatin - protected schizont cell vaccine for 20 , 000 cattle was introduced . from ningxia institute of animal husbandry and veterinary medicine against theileria annulata . on the basis of regional tests , the vaccine was applied throughout 16 townships of the county . the annual investigation showed that the safety of the vaccine was 100 % . among the 15 , 000 cattle ( including 4 , 500 water buffaloes ) inoculated with the vaccine , only 3 cattle suffered from theileriosis . the incident rate was 0 . 02 % . however of the 3600 un - inoculated cattle , 2060 heads suffered from the disease . the incident rate was 5 . 7 % . the effective protection reached 99 . 98 % with a significant social and economic effect
  河南省桐柏县是牛环形泰勒虫病流行的多发区，对养牛业危害十分严重， 1985年该县从宁夏农林科学院畜牧兽医研究所引进“虫苗” 2万头份，在区域试验的基础上在全县16个乡(镇)进行了大面积防疫注射，经年终统计表明：虫苗安全性100 % ，注苗15000头(其中有水牛4500头) ，发病3头，发病率0 . 02 % ；未注苗36000头，发病2060头，发病率5 . 7 % ，实际有效保护率达99 . 98 % ，取得了明显的社会经济效益。
• 6 . transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard . the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration , which including length of co - cultivation . those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days . they were inoculated with agrobacterium for 20 minutes . inoculated explants were co - cultivated for 4 days and in shadow at first 2 days . then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb . all of them were transferred to fresh medium every 2 weeks . the kan - resistant plants were regenerated
  芥菜外植体高频遗传转化体系的建立在最适培养基上试验了两类芥菜的三种外植体对卡那霉素的敏感性、预培养天数、浸菌时间等因素的影响，建立了芥菜高频转基因再生体系：取生长4天的芥菜子叶、下胚轴和25天的叶片在分化培养基上( ms + ba3 . 0mg / l + naa0 . 1mg / l )预培养2 - 3天后，投入农杆菌菌液中浸染20分钟，在分化培养基上暗培养2天，正常条件下培养2天后，转入抗性培养基（ ms ba3
• Three ibdv strains , designated as zj2000 , zj991 and zj992 , were isolated from bursa of fabricius of the sick chicken flocks in hangzhou , shenzhou , and ningbo of zhejiang province . the isolates could react with ibdv positive reference serum in the agar immune - diffusion test and caused 30 - 100 % mortality when passaged in chicken embryos . chickens inoculated with these isolates exhibited clinical and pathological signs typical of infectious bursal disease
  从浙江省杭州、嵊州，宁波等地暴发疑似传染性法氏囊病的鸡群中采集法氏囊病料(编号分别为zj2000 、 zj991 、 zj992 ) ，分离病毒，经琼脂免疫扩散试验、动物回归试验、鸡胚回归试验和电镜观察等证实所分离的3株野毒是传染性法氏囊病病毒。
• Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis . the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation , 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain . referred to the published sequence two primers were designed and synthesized
  给4周龄spf雏鸡人工接种鸡传染性法氏囊病病毒hb - bp毒株， 48小时后采取法氏囊，用双licl分级沉淀法提纯全长基因组dsrna ，设计一对引物，通过rt - pcr方法进行了体外扩增，获得hb株a节段基因全长cdna 。
• Acetosyringone ( as ) could dramatically improve transformation frequency , its suitable concentration is 20 mg . l " 1 . before inoculated with agrobacterium , calli should be precultured 1 ? days , and the appropriate inoculation time is 4 ~ 8 minutes , and then cocultured for 3 ~ 4days . then the cocultured calli were transferred to ms medium containing 400 mg . l " 1 cef
  7为宜；侵染前预培养1 2天，可显著提高转化效率；侵染时间以4 8分钟为宜；共培养时间3 4天较适合；转化后暗培养对转化效率没有影响；选择方法以延迟选择15天选择转化率最高。
• In order to explicit the pathogenicity differentiation of venturia inaequalis in fields , three apple cultivars were individually inoculated with 57 representative single spore isolates of venturia inaequalis collected respectively from different apple producing areas in china , england and india
  摘要为了明确田间苹果黑星病菌致病力的分化情况，选取中国、英国、印度3个国家不同苹果产区的57株有代表性的苹果黑星病菌株，分别接种嘎啦、富士、秦冠3个寄主品种进行致病力测定。
• The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination , results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm . by indirect fluorescent antibody test mdj - 01 strain was identified with tbev . a part of region encoding e protein was expanded by rt - pcr and sequenced . the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10 , respectively , which were 95 % , 94 % . mdj - 01 strain was identified with tbev again
  应用间接免疫荧光试验进行血清学鉴定，结果表明mdj - 01株为tbev 。通过rt - pcr技术扩增部分e蛋白序列并测序，在genbank上进行同源性比较，发现mdj - 01株和森张株与tbevoshima5 - 10株的同源性最高，分别为94 、 95 ，从分子生物学水平上进一步证明mdj - 01株病毒为tbev 。在鉴定的基础上，本实验对两株病毒进行了核苷酸全序列测定。
• Taken two heavy metal contaminated soils in copper mine field of hongtoushan area and lead - zinc mine field of qingchengzi area as sampling sites , studied the abundance and diversity of amf community and the relation of which to ecological factors ; two experiments were managed by pot culture and four - compartments culture system with festuca . rubra and red clover as host plants inoculated with gintraradices , gmosseae and gcaledordinum to study the effect of different amf on the uptake and accumulation of heavy metal in cd contaminated soil and cu > zn - pk cd complex - contaminated soil
  采用盆栽、四室根箱培养的方法，研究了接种g . intraradicesg . mosseae和g . caledordinum在cd污染和cu 、 zn 、 pb 、 cd复合污染土壤中对紫羊茅、三叶草吸收、累积重金属的影响，明确了丛枝菌根菌在重金属污染土壤生物修复中的作用。主要结果如下： 1 、沈阳矿区重金属污染土壤中，共发现丛枝菌根菌6个属23个种，已鉴定22种， 1个未定种。
• Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter . lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv , raise the level of serum antibody . they make chickens obtaining strong immunocompetente in a short time . agp liters of the group of infecting 0 . 4mlcbs + ibdv of germinal and 0 . 8mlcbs + ibdv of cellcular or 0 . 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely . the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method . the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv . they may also alleviate the immunological injury of activated virus to bursa of fabricius . and promote the repairation of the lesion . it can be found that bs can raise body weight gain and feed coversion ratio
  将超滤获得的法氏囊活性肽分别以不同剂量肌肉注射21日龄spf鸡，同时颈部皮下注射ibd胚毒或细胞毒灭活苗，结果表明：鸡、鸭法氏囊活性肽都能够缩短ibd油苗诱导产生抗体的时间，提高抗体水平，使鸡可以在比较短的时间内获得坚强的免疫。 0 . 4mlcbs胚毒组和0 . 8mlcbs细胞毒组或0 . 8mldbs胚毒和细胞毒组的agp抗体滴度平均比免疫对照组高2个滴度。将法氏囊活性肽与ibd活苗联合免疫鸡，结果表明：不同剂量的cbs和dbs都可以对不同日龄鸡ibd抗体的产生有不同程度的促进作用；还可以减少弱毒对鸡法氏囊组织的损伤，加快其修复。
• Steamed millets with water content of 45 % ( broomcorn ) and 42 % ( foxtail ) were inoculated with liquid culture of p . delphacis ( containing mycelial mass of ~ 25 mg / ml ) at a ratio of 20 % ( v / w ) and then incubated at 25 ? and l : d 12 : 12
  将菌丝生物量约为25mg / ml的菌丝液按20的比例( v / w )接入经高温湿热灭菌并适度熟化、含水量分别为45和42的黍米及粟米中，在25和12l ： 12d条件下直接培养，所获3 17d黍米和粟米培养物的产孢潜能和有效产孢时间因培养天数不同而异。
• Abstract : tongbai county of henan province is one of the high - epidemic areas of bovine theileriosis . in 1985 , gelatin - protected schizont cell vaccine for 20 , 000 cattle was introduced . from ningxia institute of animal husbandry and veterinary medicine against theileria annulata . on the basis of regional tests , the vaccine was applied throughout 16 townships of the county . the annual investigation showed that the safety of the vaccine was 100 % . among the 15 , 000 cattle ( including 4 , 500 water buffaloes ) inoculated with the vaccine , only 3 cattle suffered from theileriosis . the incident rate was 0 . 02 % . however of the 3600 un - inoculated cattle , 2060 heads suffered from the disease . the incident rate was 5 . 7 % . the effective protection reached 99 . 98 % with a significant social and economic effect
  文摘:河南省桐柏县是牛环形泰勒虫病流行的多发区，对养牛业危害十分严重， 1985年该县从宁夏农林科学院畜牧兽医研究所引进“虫苗” 2万头份，在区域试验的基础上在全县16个乡(镇)进行了大面积防疫注射，经年终统计表明：虫苗安全性100 % ，注苗15000头(其中有水牛4500头) ，发病3头，发病率0 . 02 % ；未注苗36000头，发病2060头，发病率5 . 7 % ，实际有效保护率达99 . 98 % ，取得了明显的社会经济效益。